Benthic macroinvertebrates are commonly used as bioindicators of water quality. In a seasonal survey of Willow Creek from 2006-2008, dissolved oxygen was low contributing to poor water quality, with consistently lower DO in upstream versus downstream portions of the creek. In this study, we investigated macroinvertebrates colonization of upstream, midstream and downstream sections of Willow creek to determine if there is biological significance associated with dissolved oxygen differences in different locations. Our results indicate that there was a higher species richness of organisms semi-sensitive or tolerant to oxygen deprivation found in the creek, with no statistical difference in species richness by location.